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I wish Geneious could do this: Look for a short sequence witin genome
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Jan 21st 2007 edited
Hi,

I would like to look for a specific combination within an imported sequence. (e.g. TCCGTCG) Is there a way I can do it and have Geneious return the location of the combination (such as position 143 within the genome)?

I am using the free version right now.

Any input will be helpful.
Jan 21st 2007
I know you can use Ctrl F to do it, but is there a way to find all those location and once?
Jan 22nd 2007
I have a similar, though expanded question. I would like to search a sequence with a large number (>300) of short sequences in one go and, ideally, have the hits mapped onto the sequence as an annotation.
Jan 23rd 2007 edited
Just a update, I still could not find the function within Geneious, hope they can add that on later because it is a very simple and useful thing to have.
Since I can not wait for them to develop the next version or a plug in, I just wrote a small program in Perl and does the job. I will make it available for download later, if any of you are interest in it.
Jan 23rd 2007
Hi,
You are right, this is a very simple and useful plugin. I have put forward the request to the development team. Though I don't think it will be out before Geneious 3.0.

If you or somebody you know has knowledge in Java you could develop this plugin for Geneious yourself using the public API.
Jan 24th 2007
I agree that this would be useful. Our group is searching for an easy-to-use platform to screen for short repeats. I think we'd purchase the 'pro' version if this was added to the next release.
Jan 24th 2007
The demand for a feature like this is become increasingly apparent. Due to popular demand we will most likely add this to version 3.0 in a couple of months time.
Jan 24th 2007
Our plan is to have the "sequence search" feature annotate all matches in the sequence. Is this the preferred behaviour?
Feb 20th 2007
One thing I noticed is that the searches are very strict, i.e. they only look for 100% matches based on the text.

Several implementations in competing software perform searches which allow one to use ambiguity symbols (N, R, Y, S, etc) for nucleotides which allows for more 'fuzzy' matching. Coming from a BioPerl background I'm not sure how hard that would be to implement here.
Feb 20th 2007
We are currently working on the ability to locate arbitrary query strings with ambiguity symbols inside a sequence document.

If instead of locating the exact locations inside a document where an ambiguous query string matches, you wanted to search for documents in your local database that match a given ambiguous query string, this would be harder to do because we maintain a search index. However, you can do a blast-like fuzzy search for documents in your local database that contain a sequence similar to your search string. To do this, click "Search" in the toolbar and then select the "Nucleotide Similarity Search" from the drop down list.
Oct 31st 2007 edited
In Geneious 3.5, a public beta of which will be released very soon, you will be able to search for subsequences including ambiguities (i.e. a motif search) in both nucleotide and amino acid sequences through the normal find dialog (Ctrl+F).
Nov 2nd 2007
Hi Guys

I think that with the growing importance and functionality of the find tool it needs to go on a tool bar somewhere.

Revel
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